Dendritic cell biocompatibility of ether-based urethane films

Authors: Ingrid Safina, Karrer M Alghazali, Luke Childress, Christopher Griffin, Ahmed Hashoosh, Ganesh Kannarpady, Fumiya Watanabe, Shawn E Bourdo, Ruud P M Dings, Alexandru S Biris, Kieng Bao Vang 

Publication: J Appl Toxicol. 2021 Jan 8. doi: 10.1002/jat.4136. Epub ahead of print. PMID: 33417269.

Abstract: The use of synthetic materials for biomedical applications is ever expanding. One of the major requirements for these materials is biocompatibility, which includes prevention of immune system responses. Due to the inherent complexity of their structural composition, the polyurethane (PU) family of polymers is being used in a variety of medical applications, from soft and hard tissue scaffolds to intricate coatings on implantable devices. Herein, we investigated whether two polymer materials, D3 and D7, induced an immune response, measured by their effects on a dendritic cell (DC) line, JAWS II. Using a lactate dehydrogenase cytotoxicity assay and Annexin V/PI staining, we found that the PU materials did not induce cytotoxicity in DC cells. Using confocal microscopy, we also showed that the materials did not induce activation or maturation, as compared to positive controls. This was confirmed by looking at various markers, CD80, CD86, MHC class I, and MHC class II, via flow cytometry. Overall, the results indicated that the investigated PU films are biocompatible in terms of immunotoxicology and immunogenicity and show great promise for use in regenerative medicine.